Taxonomic Analysis of a Haloacid Degrading Burkholderia Species Mba4 PDF Download. Download free ebook of Taxonomic Analysis of a Haloacid Degrading Burkholderia Species Mba4 in PDF format or read online by Yuen-Piu Chan,陳源標 9781361417041 Published on 2017-01-27 by Open Dissertation Press
This dissertation, |Taxonomic Analysis of a Haloacid Degrading Burkholderia Species MBA4| by Yuen-piu, Chan, 陳源標, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled TAXONOMIC ANALYSIS OF A HALOACID DEGRADING BURKHOLDERIA SPECIES MBA4 Submitted by Chan Yuen Piu for the degree of Master of Philosophy at The University of Hong Kong in August 2005 Burkholderia cepacia MBA4 was first isolated in 1985 using monobromoacetic acid as the sole carbon and energy source. The initial classification of MBA4 as Pseudomonas cepacia and subsequently as Burkholderia cepacia was based mainly on simple biochemical and substrate utilization tests. It was later found that these tests alone cannot accurately differentiate all the species within the Burkholderia genus. The application of newly developed molecular techniques has greatly changed the classification of the Burkholderia species. Several bacteria that were originally identified as B. cepacia have been reclassified as novel Burkholderia species. In this study, MBA4 was subjected to taxonomic analysis through a polyphasic approach, in which phenotypical, genotypical, and phylogenetical information were gathered to achieve a consensus classification. API 20NE and BIOLOG GN MicroPlate analysis results confirmed that MBA4 is a member of the Burkholderia genus. In addition, whole cell fatty acid and whole cell protein SDS-PAGE profiles also indicated possible Burkholderia species that are closely related to MBA4. PCR primer pairs recAF3/recAB3 and recAF2/recXB2 have been used to amplify the recA genes and the recA-recX junctions from MBA4 and nine other Burkholderia species. The DNA sequences of the amplified products have been determined. Phylogenetic analysis of the 16S rRNA and the recA genes showed that MBA4 is most closely related to B. caribensis, followed by B. hospita and B. phymatum. BOX-PCR fingerprinting analysis showed that the genome structure of MBA4 is different from that of other Burkholderia species. The DNA-DNA hybridization value between MBA4 and B. caribensis is 74%, which is higher than the recommended value for species delineation. The results of polyphasic analysis concluded that MBA4 is an atypical strain of B. caribensis. DOI: 10.5353/th_b3609637 Subjects: Ralstonia Bacteria - Analysis
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